Biochemistry’s From the Bench manuscripts are designed to report detailed protocols of high interest to members of the biological chemistry community.
Learn more about Biochemistry’s From the Bench articles in this editorial announcement.
Browse Recent Articles
Analysis of Tagged Proteins Using Tandem Affinity-Buffer Exchange Chromatography Online with Native Mass Spectrometry
Biochemistry 2021 60 (24), 1876-1884
DOI: 10.1021/acs.biochem.1c00138
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Modeling Immunity with Rosetta: Methods for Antibody and Antigen Design
Biochemistry 2021 60 (11), 825-846
DOI: 10.1021/acs.biochem.0c00912
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Capturing Membrane Protein Ribosome Nascent Chain Complexes in a Native-like Environment for Co-translational Studies
Biochemistry 2020 59 (30), 2764-2775
DOI: 10.1021/acs.biochem.0c00423
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Monitoring Protein–Protein Interactions in the Cyanobacterial Circadian Clock in Real Time via Electron Paramagnetic Resonance Spectroscopy
Biochemistry 2020 59 (26), 2387-2400
DOI: 10.1021/acs.biochem.0c00279
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Urea-Assisted Reconstitution of Discoidal High-Density Lipoprotein
Biochemistry 2020 59 (15), 1455-1464
DOI: 10.1021/acs.biochem.0c00075
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Rheostatic Control of Protein Expression Using Tuner Cells
Biochemistry 2020 59 (6), 733-735
DOI: 10.1021/acs.biochem.9b01101
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One-Atom Substitution Enables Direct and Continuous Monitoring of Histone Deacylase Activity
Biochemistry 2019 58 (48), 4777-4789
DOI: 10.1021/acs.biochem.9b00786
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An Improved Method for Bacterial Immunofluorescence Staining To Eliminate Antibody Exclusion from the Fixed Nucleoid
Biochemistry 2019 58 (45), 4457-4465
DOI: 10.1021/acs.biochem.9b00724
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The EFI Web Resource for Genomic Enzymology Tools: Leveraging Protein, Genome, and Metagenome Databases to Discover Novel Enzymes and Metabolic Pathways
Biochemistry 2019 58 (41), 4169-4182
DOI: 10.1021/acs.biochem.9b00735
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Scalable Chemoenzymatic Synthesis of Inositol Pyrophosphates
Biochemistry 2019 58 (38), 3927-3932
DOI: 10.1021/acs.biochem.9b00587
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Guidelines for SHAPE Reagent Choice and Detection Strategy for RNA Structure Probing Studies
Biochemistry 2019 58 (23), 2655-2664
DOI: 10.1021/acs.biochem.8b01218
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Dihedral Angle Calculations To Elucidate the Folding of Peptides through Its Main Mechanical Forces
Biochemistry 2019 58 (8), 1032-1037
DOI: 10.1021/acs.biochem.8b01101
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A Fluorogenic Assay To Monitor Rho-Dependent Termination of Transcription
Biochemistry 2019 58 (7), 865-874
DOI: 10.1021/acs.biochem.8b01216
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An Open-Source Plate Reader
Biochemistry 2019 58 (6), 468-473
DOI: 10.1021/acs.biochem.8b00952
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A Sensitive, Nonradioactive Assay for Zn(II) Uptake into Metazoan Cells
Biochemistry 2018 57 (50), 6807-6815
DOI: 10.1021/acs.biochem.8b01043
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An Improved Strategy for Fluorescent Tagging of Membrane Proteins for Overexpression and Purification in Mammalian Cells
Biochemistry 2018 57 (49), 6741-6751
DOI: 10.1021/acs.biochem.8b01070
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Optimization of Chemoenzymatic Mass Tagging by Strain-Promoted Cycloaddition (SPAAC) for the Determination of O-GlcNAc Stoichiometry by Western Blotting
Biochemistry 2018 57 (40), 5769-5774
DOI: 10.1021/acs.biochem.8b00648
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Detection of Inosine on Transfer RNAs without a Reverse Transcription Reaction
Biochemistry 2018 57 (39), 5641-5647
DOI: 10.1021/acs.biochem.8b00718
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Evolution of a Thermophilic Strand-Displacing Polymerase Using High-Temperature Isothermal Compartmentalized Self-Replication
Biochemistry 2018 57 (31), 4607-4619
DOI: 10.1021/acs.biochem.8b00200
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Improved Method for the Incorporation of Heme Cofactors into Recombinant Proteins Using Escherichia coli Nissle 1917
Biochemistry 2018 57 (19), 2747-2755
DOI: 10.1021/acs.biochem.8b00242
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pytc: Open-Source Python Software for Global Analyses of Isothermal Titration Calorimetry Data
Biochemistry 2018 57 (18), 2578-2583
DOI: 10.1021/acs.biochem.7b01264
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A Novel Domain Assembly Routine for Creating Full-Length Models of Membrane Proteins from Known Domain Structures
Biochemistry 2018 57 (13), 1939-1944
DOI: 10.1021/acs.biochem.7b00995
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Membrane Protein Structure in Live Cells: Methodology for Studying Drug Interaction by Mass Spectrometry-Based Footprinting
Biochemistry 2018 57 (3), 286-294
DOI: 10.1021/acs.biochem.7b00874
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An Automated Image Analysis Method for Segmenting Fluorescent Bacteria in Three Dimensions
Biochemistry 2018 57 (2), 209-215
DOI: 10.1021/acs.biochem.7b00839
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Generation and Characterization of Recombinant Antibody-like ADP-Ribose Binding Proteins
Biochemistry 2017 56 (48), 6305-6316
DOI: 10.1021/acs.biochem.7b00670
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Thermal Shift as an Entropy-Driven Effect
Biochemistry 2017 56 (47), 6187-6199
DOI: 10.1021/acs.biochem.7b00860
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Quantification of Active Apohemoglobin Heme-Binding Sites via Dicyanohemin Incorporation
Biochemistry 2017 56 (40), 5245-5259
DOI: 10.1021/acs.biochem.7b00683
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A Multimodal Spectroscopic Imaging Method To Characterize the Metal and Macromolecular Content of Proteinaceous Aggregates (“Amyloid Plaques”)
Biochemistry 2017 56 (32), 4107-4116
DOI: 10.1021/acs.biochem.7b00262
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Detection of a Subset of Posttranscriptional Transfer RNA Modifications in Vivo with a Restriction Fragment Length Polymorphism-Based Method
Biochemistry 2017 56 (31), 4029-4038
DOI: 10.1021/acs.biochem.7b00324
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Methylated Cytosine Maintains G-Quadruplex Structures during Polymerase Chain Reaction and Contributes to Allelic Dropout
Biochemistry 2017 56 (29), 3691-3698
DOI: 10.1021/acs.biochem.7b00480
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Monitoring the Retention of Human Proliferating Cell Nuclear Antigen at Primer/Template Junctions by Proteins That Bind Single-Stranded DNA
Biochemistry 2017 56 (27), 3415-3421
DOI: 10.1021/acs.biochem.7b00386
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Laser-Induced Population Inversion in Rhodamine 6G for Lysozyme Oligomer Detection
Biochemistry 2017 56 (22), 2762-2765
DOI: 10.1021/acs.biochem.7b00243
