Biochemistry’s From the Bench manuscripts are designed to report detailed protocols of high interest to members of the biological chemistry community.
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Learn more about Biochemistry’s From the Bench articles in this editorial announcement.
Browse Recent Articles
Analysis of Tagged Proteins Using Tandem Affinity-Buffer Exchange Chromatography Online with Native Mass Spectrometry
Biochemistry 2021 60 (24), 1876-1884
DOI: 10.1021/acs.biochem.1c00138
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Modeling Immunity with Rosetta: Methods for Antibody and Antigen Design
Biochemistry 2021 60 (11), 825-846
DOI: 10.1021/acs.biochem.0c00912
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Capturing Membrane Protein Ribosome Nascent Chain Complexes in a Native-like Environment for Co-translational Studies
Biochemistry 2020 59 (30), 2764-2775
DOI: 10.1021/acs.biochem.0c00423
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Monitoring Protein–Protein Interactions in the Cyanobacterial Circadian Clock in Real Time via Electron Paramagnetic Resonance Spectroscopy
Biochemistry 2020 59 (26), 2387-2400
DOI: 10.1021/acs.biochem.0c00279
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Urea-Assisted Reconstitution of Discoidal High-Density Lipoprotein
Biochemistry 2020 59 (15), 1455-1464
DOI: 10.1021/acs.biochem.0c00075
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Rheostatic Control of Protein Expression Using Tuner Cells
Biochemistry 2020 59 (6), 733-735
DOI: 10.1021/acs.biochem.9b01101
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One-Atom Substitution Enables Direct and Continuous Monitoring of Histone Deacylase Activity
Biochemistry 2019 58 (48), 4777-4789
DOI: 10.1021/acs.biochem.9b00786
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An Improved Method for Bacterial Immunofluorescence Staining To Eliminate Antibody Exclusion from the Fixed Nucleoid
Biochemistry 2019 58 (45), 4457-4465
DOI: 10.1021/acs.biochem.9b00724
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The EFI Web Resource for Genomic Enzymology Tools: Leveraging Protein, Genome, and Metagenome Databases to Discover Novel Enzymes and Metabolic Pathways
Biochemistry 2019 58 (41), 4169-4182
DOI: 10.1021/acs.biochem.9b00735
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Scalable Chemoenzymatic Synthesis of Inositol Pyrophosphates
Biochemistry 2019 58 (38), 3927-3932
DOI: 10.1021/acs.biochem.9b00587
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Guidelines for SHAPE Reagent Choice and Detection Strategy for RNA Structure Probing Studies
Biochemistry 2019 58 (23), 2655-2664
DOI: 10.1021/acs.biochem.8b01218
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Dihedral Angle Calculations To Elucidate the Folding of Peptides through Its Main Mechanical Forces
Biochemistry 2019 58 (8), 1032-1037
DOI: 10.1021/acs.biochem.8b01101
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A Fluorogenic Assay To Monitor Rho-Dependent Termination of Transcription
Biochemistry 2019 58 (7), 865-874
DOI: 10.1021/acs.biochem.8b01216
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An Open-Source Plate Reader
Biochemistry 2019 58 (6), 468-473
DOI: 10.1021/acs.biochem.8b00952
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A Sensitive, Nonradioactive Assay for Zn(II) Uptake into Metazoan Cells
Biochemistry 2018 57 (50), 6807-6815
DOI: 10.1021/acs.biochem.8b01043
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An Improved Strategy for Fluorescent Tagging of Membrane Proteins for Overexpression and Purification in Mammalian Cells
Biochemistry 2018 57 (49), 6741-6751
DOI: 10.1021/acs.biochem.8b01070
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Optimization of Chemoenzymatic Mass Tagging by Strain-Promoted Cycloaddition (SPAAC) for the Determination of O-GlcNAc Stoichiometry by Western Blotting
Biochemistry 2018 57 (40), 5769-5774
DOI: 10.1021/acs.biochem.8b00648
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Detection of Inosine on Transfer RNAs without a Reverse Transcription Reaction
Biochemistry 2018 57 (39), 5641-5647
DOI: 10.1021/acs.biochem.8b00718
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Evolution of a Thermophilic Strand-Displacing Polymerase Using High-Temperature Isothermal Compartmentalized Self-Replication
Biochemistry 2018 57 (31), 4607-4619
DOI: 10.1021/acs.biochem.8b00200
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Improved Method for the Incorporation of Heme Cofactors into Recombinant Proteins Using Escherichia coli Nissle 1917
Biochemistry 2018 57 (19), 2747-2755
DOI: 10.1021/acs.biochem.8b00242
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pytc: Open-Source Python Software for Global Analyses of Isothermal Titration Calorimetry Data
Biochemistry 2018 57 (18), 2578-2583
DOI: 10.1021/acs.biochem.7b01264
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A Novel Domain Assembly Routine for Creating Full-Length Models of Membrane Proteins from Known Domain Structures
Biochemistry 2018 57 (13), 1939-1944
DOI: 10.1021/acs.biochem.7b00995
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Membrane Protein Structure in Live Cells: Methodology for Studying Drug Interaction by Mass Spectrometry-Based Footprinting
Biochemistry 2018 57 (3), 286-294
DOI: 10.1021/acs.biochem.7b00874
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An Automated Image Analysis Method for Segmenting Fluorescent Bacteria in Three Dimensions
Biochemistry 2018 57 (2), 209-215
DOI: 10.1021/acs.biochem.7b00839
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Generation and Characterization of Recombinant Antibody-like ADP-Ribose Binding Proteins
Biochemistry 2017 56 (48), 6305-6316
DOI: 10.1021/acs.biochem.7b00670
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Thermal Shift as an Entropy-Driven Effect
Biochemistry 2017 56 (47), 6187-6199
DOI: 10.1021/acs.biochem.7b00860
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Quantification of Active Apohemoglobin Heme-Binding Sites via Dicyanohemin Incorporation
Biochemistry 2017 56 (40), 5245-5259
DOI: 10.1021/acs.biochem.7b00683
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A Multimodal Spectroscopic Imaging Method To Characterize the Metal and Macromolecular Content of Proteinaceous Aggregates (“Amyloid Plaques”)
Biochemistry 2017 56 (32), 4107-4116
DOI: 10.1021/acs.biochem.7b00262
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Detection of a Subset of Posttranscriptional Transfer RNA Modifications in Vivo with a Restriction Fragment Length Polymorphism-Based Method
Biochemistry 2017 56 (31), 4029-4038
DOI: 10.1021/acs.biochem.7b00324
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Methylated Cytosine Maintains G-Quadruplex Structures during Polymerase Chain Reaction and Contributes to Allelic Dropout
Biochemistry 2017 56 (29), 3691-3698
DOI: 10.1021/acs.biochem.7b00480
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Monitoring the Retention of Human Proliferating Cell Nuclear Antigen at Primer/Template Junctions by Proteins That Bind Single-Stranded DNA
Biochemistry 2017 56 (27), 3415-3421
DOI: 10.1021/acs.biochem.7b00386
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Laser-Induced Population Inversion in Rhodamine 6G for Lysozyme Oligomer Detection
Biochemistry 2017 56 (22), 2762-2765
DOI: 10.1021/acs.biochem.7b00243